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invivo mab rat igg2a isotype control antibody clone 2a3  (Bio X Cell)


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    Bio X Cell invivo mab rat igg2a isotype control antibody clone 2a3
    Invivo Mab Rat Igg2a Isotype Control Antibody Clone 2a3, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/igg2a+isotype+control+2a3+antibody/anti+cd4+gk1+5/pmc12255530-46-0-10
    Average 90 stars, based on 1 article reviews
    invivo mab rat igg2a isotype control antibody clone 2a3 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Control:

    Article Title: Acyl-CoA-binding protein (ACBP): a poor-prognosis biomarker in sepsis and a target for disease mitigation
    Article Snippet: .. Neutrophil depletion was achieved by intraperitoneal injections of anti-mouse Ly6G antibody (clone 1A8, 50 μg/mouse, #BE0075-1, BioXcell) or isotype IgG2a control (clone 2A3, #BE0089, BioXcell) at 36 h and 12 h before E. coli injection., Additionally, α-ACBP (2.5 mg/kg) was administered twice more in the hours preceding E. coli challenge. ..

    Article Title: Targeting PAR-2 with a negative allosteric modulator increases tumor antigen presentation and potentiates anti-PD-1 immunotherapy.
    Article Snippet: PaB670129 was synthesized according to US20180305450A1 by RD- Biotech. .. Anti- mouse PD1 (clone RMP1- 14, cat. BE0146, BioXCell, RRID: AB_10949053), anti- mouse IgG2a isotype control (clone 2A3, cat. BE0089, BioXCell, RRID:AB_1107769), I- 117 was synthesized according to WO2018057588 by Viva Biotech and provided by Domain Therapeutics; PAR1 antagonist RWJ 56110 (cat. 2614) from Tocris; Hydroxypropyl methylcellulose (HPMC, Sigma- Aldrich), hydroxypropyl- beta- cyclodextrin (HpBCD, ChemCruz), anhydrous Ethyl Alcohol (Eth, Commercial alcohols), and polyethylene glycol 400 (PEG 400, cat. P5402, Sigma- Aldrich). .. Deoxyribonuclease I (cat. 9003- 98- 9 Worthington), collagenase A (cat. 9001- 12- 1, Worthington), RPMI 1640 (cat. 350- 000 RL, Wisent), PBS (cat. 311- 013 CL, Wisent), EDTA (cat. 15575020, Invitrogen), CD16/CD32 (cat. 70- 0161 M00, TONBO Biosciences), brilliant stain buffer (cat. 566349, BD Biosciences), FoxP3 Fixation/Permeabilization Buffer Set (cat. 00- 5521- 00, Invitrogen), and TRIzol (cat. 15596026, Invitrogen).

    Article Title: ROCK1 inhibition primes anti-tumor immunity in EGFR-mutant NSCLC by triggering PD-L1 degradation mediated by SMURF2.
    Article Snippet: .. Experimental Section/Methods Reagents and antibodies: The compounds used in this study are listed below with their sources: Y-27632 (HY-10583, MedChemExpress), fasudil (HY-10341, MedChemExpress), LY-2090314 (HY-16294, MedChemExpress), MG-132 (HY-13259, MedChemExpress), human recombinant IL-2 (200-02-50UG, Peprotech), anti-mouse PD-L1 (BE0101, BioXCell), and isotype control IgG (BE0089, BioXCell). .. The following antibodies were used in this study: BV605 anti-human PD-L1 (329724, Biolegend), Zombie Aqua Fixable Viability Kit (423102, Biolegend), APC/Cy7 anti-mouse CD45 (103116, Biolegend), BV785 anti-mouse CD3 (100231, Biolegend), Percp-cy5.5 anti-mouse CD4 (100434, Biolegend), AR TIC LE IN PR ES S BV711 anti-mouse CD8 (100747, Biolegend), APC anti-mouse Granzyme B (372204, Biolegend), AF700 anti-mouse Ki-67 (652420, Biolegend), BV650 anti-mouse IFNγ (505832, Biolegend), APC/Cy7 anti-human CD45 (304014, Biolegend), Percp-cy5.5 anti-human CD4 (300530, Biolegend), BV785 anti-human CD8 (344740, Biolegend), AF700 anti-human GZMB (372222, Biolegend), BV650 anti-human IFNγ (505546, Biolegend), BV711 anti-human Ki-67 (350516, Biolegend), PE/Cy7 anti-mouse TIM-3 (119715, Biolegend), BV605 anti-mouse PD-1 (135219, Biolegend), BV421 anti-mouse CD25 (113705, Biolegend), PE anti-mouse FOXP3 (118903, Biolegend), PE/Cy7 anti-mouse F4/80 (123113, Biolegend), PE anti-mouse CD11b (101207, Biolegend), BV650 anti-mouse Ly6G (127641, Biolegend), AF700 anti-mouse Ly6C (128023, Biolegend), BV421 anti- mouse CD80 (104725, Biolegend), BV785 anti-mouse CD206 (141729, Biolegend), BV711 anti-mouse CD11c (117349, Biolegend), ROCK1 (4035, Cell Signaling Technology), PD-L1 (13684, Cell Signaling Technology), PD-L1 (66248, Proteintech), β-actin (66009, Proteintech), GAPDH (ab8245, Abcam), Phospho-Ser/Thr (9631, Cell Signaling Technology), Phospho PD-L1 (S283) (AP1270, Abclonal), GSK3α (sc-5264, Santa Cruz), GSK3β (sc-81462, Santa Cruz), Phospho GSK3α (S21) (AF3336, Affinity), Phospho GSK3β (S9) (AF2016, Affinity), Ub (P4D1) (sc-8017, Santa Cruz), SMURF2 (12024, Cell Signaling Technology), Myc-Tag (2276, Cell Signaling Technology), FLAG-Tag (2368, Cell Signaling Technology), Anti- Rabbit IgG, horseradish peroxidase (HRP)-linked (7074, Cell Signaling Technology), Anti-Mouse IgG, HRP-linked (7076, Cell Signaling Technology), Rabbit Anti-Mouse IgG (Light Chain Specific) (58802, Cell AR TIC LE IN PR ES S Signaling Technology), Mouse Anti-Rabbit IgG (Conformation Specific) (5127, Cell Signaling Technology), and Phospho-ROCK1 (T455+S456) (ab203273, Abcam).

    Article Title: Chloride channel accessory 4 suppresses stem cell-like properties of colorectal cancer and enhances anti-PD-1 immunotherapy
    Article Snippet: .. After 7 days, mice were intraperitoneally treated with either an in vivo blocking antibody against mouse PD-1 (Clone: 29F.1A2, BioXcell, Cat# BP0273) or a rat IgG2a isotype control antibody (Clone: 2A3, BioXcell, Cat# BP0089). ..

    Article Title: Targeting Mettl8-Tcf1 axis promotes CD8 + T PEX differentiation and antitumor immunity
    Article Snippet: .. Anti–PD-1 treatments involved i.p. administration of 200 μg per mouse of anti–PD-1 antibody (clone RMP1-14, cat. no. A2122; Selleckchem) or rat IgG2a isotype control (clone 2A3, cat. no. BE0089; Bio X Cell) in 200 μL PBS every 2 days from day 11 to 17 or day 12 to 18 after inoculation. ..

    Article Title: Lymphocyte Antigen 6G Mediates Vagotomy‐Associated Reduction in Body Weight
    Article Snippet: Nuclei count was measured in ImageJ FIJI by threshold and particles counting, then normalized by the following equation: Non‐adipocyte nuclei % = ((Total nuclei count—Adipocyte count)/Total nuclei count) × 100%. .. To ligate Ly6G surface epitopes, male C57BL/6 mice were intraperitoneally injected with InVivo Mab anti‐mouse Ly6G (100 ug/100 uL) (Bioxcell, #BE0075) or for control InVivoMAb rat IgG2a (anti‐trinitrophenol) isotype (100 ug/100 uL) (Bioxcell, #BE0089) antibodies 2 days before the sham or VX surgery. ..

    Article Title: Chloride channel accessory 4 suppresses stem cell-like properties of colorectal cancer and enhances anti-PD-1 immunotherapy
    Article Snippet: .. Beginning on day 7 post-surgery, they were then administered an in vivo blocking antibody against mouse PD-1 (Clone: 29F.1A2, sourced from BioXcell, Cat# BP0273) or a rat IgG2a isotype control antibody (Clone: 2A3, from BioXcell, Cat# BP0089) via intraperitoneal injection. ..

    Article Title: Lymphocyte Antigen 6G Mediates Vagotomy‐Associated Reduction in Body Weight
    Article Snippet: Nuclei count was measured in ImageJ FIJI by threshold and particles counting, then normalized by the following equation: Non- adipocyte nuclei % = ((Total nuclei count—Adipocyte count)/Total nuclei count) × 100%. .. To ligate Ly6G surface epitopes, male C57BL/6 mice were intraperitoneally injected with InVivo Mab anti- mouse Ly6G (100 ug/100 uL) (Bioxcell, #BE0075) or for control InVivoMAb rat IgG2a (anti- trinitrophenol) isotype (100 ug/100 uL) (Bioxcell, #BE0089) antibodies 2 days before the sham or VX surgery. ..

    Injection:

    Article Title: Acyl-CoA-binding protein (ACBP): a poor-prognosis biomarker in sepsis and a target for disease mitigation
    Article Snippet: .. Neutrophil depletion was achieved by intraperitoneal injections of anti-mouse Ly6G antibody (clone 1A8, 50 μg/mouse, #BE0075-1, BioXcell) or isotype IgG2a control (clone 2A3, #BE0089, BioXcell) at 36 h and 12 h before E. coli injection., Additionally, α-ACBP (2.5 mg/kg) was administered twice more in the hours preceding E. coli challenge. ..

    Article Title: Lymphocyte Antigen 6G Mediates Vagotomy‐Associated Reduction in Body Weight
    Article Snippet: Nuclei count was measured in ImageJ FIJI by threshold and particles counting, then normalized by the following equation: Non‐adipocyte nuclei % = ((Total nuclei count—Adipocyte count)/Total nuclei count) × 100%. .. To ligate Ly6G surface epitopes, male C57BL/6 mice were intraperitoneally injected with InVivo Mab anti‐mouse Ly6G (100 ug/100 uL) (Bioxcell, #BE0075) or for control InVivoMAb rat IgG2a (anti‐trinitrophenol) isotype (100 ug/100 uL) (Bioxcell, #BE0089) antibodies 2 days before the sham or VX surgery. ..

    Article Title: Chloride channel accessory 4 suppresses stem cell-like properties of colorectal cancer and enhances anti-PD-1 immunotherapy
    Article Snippet: .. Beginning on day 7 post-surgery, they were then administered an in vivo blocking antibody against mouse PD-1 (Clone: 29F.1A2, sourced from BioXcell, Cat# BP0273) or a rat IgG2a isotype control antibody (Clone: 2A3, from BioXcell, Cat# BP0089) via intraperitoneal injection. ..

    Article Title: Lymphocyte Antigen 6G Mediates Vagotomy‐Associated Reduction in Body Weight
    Article Snippet: Nuclei count was measured in ImageJ FIJI by threshold and particles counting, then normalized by the following equation: Non- adipocyte nuclei % = ((Total nuclei count—Adipocyte count)/Total nuclei count) × 100%. .. To ligate Ly6G surface epitopes, male C57BL/6 mice were intraperitoneally injected with InVivo Mab anti- mouse Ly6G (100 ug/100 uL) (Bioxcell, #BE0075) or for control InVivoMAb rat IgG2a (anti- trinitrophenol) isotype (100 ug/100 uL) (Bioxcell, #BE0089) antibodies 2 days before the sham or VX surgery. ..

    Synthesized:

    Article Title: Targeting PAR-2 with a negative allosteric modulator increases tumor antigen presentation and potentiates anti-PD-1 immunotherapy.
    Article Snippet: PaB670129 was synthesized according to US20180305450A1 by RD- Biotech. .. Anti- mouse PD1 (clone RMP1- 14, cat. BE0146, BioXCell, RRID: AB_10949053), anti- mouse IgG2a isotype control (clone 2A3, cat. BE0089, BioXCell, RRID:AB_1107769), I- 117 was synthesized according to WO2018057588 by Viva Biotech and provided by Domain Therapeutics; PAR1 antagonist RWJ 56110 (cat. 2614) from Tocris; Hydroxypropyl methylcellulose (HPMC, Sigma- Aldrich), hydroxypropyl- beta- cyclodextrin (HpBCD, ChemCruz), anhydrous Ethyl Alcohol (Eth, Commercial alcohols), and polyethylene glycol 400 (PEG 400, cat. P5402, Sigma- Aldrich). .. Deoxyribonuclease I (cat. 9003- 98- 9 Worthington), collagenase A (cat. 9001- 12- 1, Worthington), RPMI 1640 (cat. 350- 000 RL, Wisent), PBS (cat. 311- 013 CL, Wisent), EDTA (cat. 15575020, Invitrogen), CD16/CD32 (cat. 70- 0161 M00, TONBO Biosciences), brilliant stain buffer (cat. 566349, BD Biosciences), FoxP3 Fixation/Permeabilization Buffer Set (cat. 00- 5521- 00, Invitrogen), and TRIzol (cat. 15596026, Invitrogen).

    Recombinant:

    Article Title: ROCK1 inhibition primes anti-tumor immunity in EGFR-mutant NSCLC by triggering PD-L1 degradation mediated by SMURF2.
    Article Snippet: .. Experimental Section/Methods Reagents and antibodies: The compounds used in this study are listed below with their sources: Y-27632 (HY-10583, MedChemExpress), fasudil (HY-10341, MedChemExpress), LY-2090314 (HY-16294, MedChemExpress), MG-132 (HY-13259, MedChemExpress), human recombinant IL-2 (200-02-50UG, Peprotech), anti-mouse PD-L1 (BE0101, BioXCell), and isotype control IgG (BE0089, BioXCell). .. The following antibodies were used in this study: BV605 anti-human PD-L1 (329724, Biolegend), Zombie Aqua Fixable Viability Kit (423102, Biolegend), APC/Cy7 anti-mouse CD45 (103116, Biolegend), BV785 anti-mouse CD3 (100231, Biolegend), Percp-cy5.5 anti-mouse CD4 (100434, Biolegend), AR TIC LE IN PR ES S BV711 anti-mouse CD8 (100747, Biolegend), APC anti-mouse Granzyme B (372204, Biolegend), AF700 anti-mouse Ki-67 (652420, Biolegend), BV650 anti-mouse IFNγ (505832, Biolegend), APC/Cy7 anti-human CD45 (304014, Biolegend), Percp-cy5.5 anti-human CD4 (300530, Biolegend), BV785 anti-human CD8 (344740, Biolegend), AF700 anti-human GZMB (372222, Biolegend), BV650 anti-human IFNγ (505546, Biolegend), BV711 anti-human Ki-67 (350516, Biolegend), PE/Cy7 anti-mouse TIM-3 (119715, Biolegend), BV605 anti-mouse PD-1 (135219, Biolegend), BV421 anti-mouse CD25 (113705, Biolegend), PE anti-mouse FOXP3 (118903, Biolegend), PE/Cy7 anti-mouse F4/80 (123113, Biolegend), PE anti-mouse CD11b (101207, Biolegend), BV650 anti-mouse Ly6G (127641, Biolegend), AF700 anti-mouse Ly6C (128023, Biolegend), BV421 anti- mouse CD80 (104725, Biolegend), BV785 anti-mouse CD206 (141729, Biolegend), BV711 anti-mouse CD11c (117349, Biolegend), ROCK1 (4035, Cell Signaling Technology), PD-L1 (13684, Cell Signaling Technology), PD-L1 (66248, Proteintech), β-actin (66009, Proteintech), GAPDH (ab8245, Abcam), Phospho-Ser/Thr (9631, Cell Signaling Technology), Phospho PD-L1 (S283) (AP1270, Abclonal), GSK3α (sc-5264, Santa Cruz), GSK3β (sc-81462, Santa Cruz), Phospho GSK3α (S21) (AF3336, Affinity), Phospho GSK3β (S9) (AF2016, Affinity), Ub (P4D1) (sc-8017, Santa Cruz), SMURF2 (12024, Cell Signaling Technology), Myc-Tag (2276, Cell Signaling Technology), FLAG-Tag (2368, Cell Signaling Technology), Anti- Rabbit IgG, horseradish peroxidase (HRP)-linked (7074, Cell Signaling Technology), Anti-Mouse IgG, HRP-linked (7076, Cell Signaling Technology), Rabbit Anti-Mouse IgG (Light Chain Specific) (58802, Cell AR TIC LE IN PR ES S Signaling Technology), Mouse Anti-Rabbit IgG (Conformation Specific) (5127, Cell Signaling Technology), and Phospho-ROCK1 (T455+S456) (ab203273, Abcam).

    In Vivo:

    Article Title: Chloride channel accessory 4 suppresses stem cell-like properties of colorectal cancer and enhances anti-PD-1 immunotherapy
    Article Snippet: .. After 7 days, mice were intraperitoneally treated with either an in vivo blocking antibody against mouse PD-1 (Clone: 29F.1A2, BioXcell, Cat# BP0273) or a rat IgG2a isotype control antibody (Clone: 2A3, BioXcell, Cat# BP0089). ..

    Article Title: Chloride channel accessory 4 suppresses stem cell-like properties of colorectal cancer and enhances anti-PD-1 immunotherapy
    Article Snippet: .. Beginning on day 7 post-surgery, they were then administered an in vivo blocking antibody against mouse PD-1 (Clone: 29F.1A2, sourced from BioXcell, Cat# BP0273) or a rat IgG2a isotype control antibody (Clone: 2A3, from BioXcell, Cat# BP0089) via intraperitoneal injection. ..

    Blocking Assay:

    Article Title: Chloride channel accessory 4 suppresses stem cell-like properties of colorectal cancer and enhances anti-PD-1 immunotherapy
    Article Snippet: .. After 7 days, mice were intraperitoneally treated with either an in vivo blocking antibody against mouse PD-1 (Clone: 29F.1A2, BioXcell, Cat# BP0273) or a rat IgG2a isotype control antibody (Clone: 2A3, BioXcell, Cat# BP0089). ..

    Article Title: Chloride channel accessory 4 suppresses stem cell-like properties of colorectal cancer and enhances anti-PD-1 immunotherapy
    Article Snippet: .. Beginning on day 7 post-surgery, they were then administered an in vivo blocking antibody against mouse PD-1 (Clone: 29F.1A2, sourced from BioXcell, Cat# BP0273) or a rat IgG2a isotype control antibody (Clone: 2A3, from BioXcell, Cat# BP0089) via intraperitoneal injection. ..



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